Mass Spectrometry

by Richard Caprioli and Marc Sutter, revised by Markus Stoeckli

Parts of this tutorial were published in:

'Introduction to Biophysical Methods for Protein and Nucleic Acid Research', J. A. Glasel and Murray P. Deutscher, editors, chapter 4, Academic Press 1995.

 

Table of Contents

 

1. Introduction

2. Instrumentation

2.1. Basic Concepts

2.2. Ionization

2.2.1. Electron Impact and Chemical Ionization

2.2.2. Plasma Desorption Mass Spectroscopy (PDMS)

2.2.3. Fast Atom Bombardment (FAB)

2.2.4. Thermospray and Particle Beam

2.2.5. Electrospray (ES)

2.2.6. Matrix-Assisted Laser Desorption (MALD)

2.3. Mass Analysis

2.3.1. Sector Instruments

2.3.2. Quadrupole Mass Filter and Quadrupole Ion Trap Instruments

2.3.3. Time-of-Flight

2.3.4. Ion Cyclotron Resonance Mass Spectrometry

3. Combined Techniques

3.1. Tandem Mass Spectrometry

3.2. LC/MS

3.3. Capillary electrophoresis/MS

4. Applications

4.1. Determination of Molecular Mass

4.2. Peptides

4.2.1. Choice of Ionization Technique

4.2.2. Mixture Analysis

4.2.3. Sequence Analysis

4.2.4. Synthetic Peptides

4.2.5. Sequence Ordered Tryptic Maps

4.3. Proteins

4.3.1. Molecular Mass

4.3.2. Post-Translational Modifications

4.3.3. Location of Disulfide Linkages

4.3.4. Non-Covalent Interactions

4.4. Nucleic Acids

4.4.1. Nucleosides, Nucleotides, and Oligonucleotides

4.4.2. Oligonucleotides

5. Conclusions

6. Glossary

7. References