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Mass spectrum of insulin

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Cleavages Observed in MS/MS of Peptides

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PROBLEM

	Bank President
	Who robbed the bank?
	Biologist
	What protein was isolated?

GATHER EVIDENCE

	Mass Spectrometrist
	1.  Interview biologist who isolated the protein
	2.  Cleave protein to obtain peptide mixture
	3. Analyze peptide mixture by MS to obtain peptide molecular masses!
	Police Officer
	1. Interview witnesses
	2. Dust for fingerprints

DATABASE SEARCH

	Police Officer
	Height:  5’7”
	Weight:  160 lbs
	Gender:  male
	Age:  35-40
	Fingerprints
	Mass Spectrometrist
	Approx. molecular weight:  30,000
	Origin:  bovine liver
	Peptide mass list from MS analysis:  975.4832, 1112.5368, 632.3147, 803.4134, 764.3892

DATABASE SEARCH RESULTS

	Police Officer
	Identifies the robber
	Anthony J. Felon
	Mass Spectrometrist
	Identifies the protein
	bovine carbonic anhydrase

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