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Built-in PhosphorImager system
functionality
Although
nonradioactive methods are increasing in
popularity, many gel and blot studies involving
specific detection of DNA or protein still use radiolabeled
probes. Some applications, such as metabolic uptake assays, will continue
to require the use of radioisotopes. So the Storm system has fast, filmless, PhosphorImager
system functionality built in to handle these applications. Now, even as
you adopt new non-radioactive alternatives, you can continue to use popular
radioisotope labeling and detection methods.
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Fig 1. With five orders of linear dynamic range, Storm
captures the image from both strong and weak signals in a single
exposure. The Storm system's linear dynamic range is 1000 times greater
than film.
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Publication-ready
data in one-tenth the time
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Southern, Northern, Western, and other radio-labeled
blotting assays produce images on storage phosphor screens 10-100 times
faster than with film. And Storm lets you choose any radioisotope (or nonradioactive chemifluorescence)
for blotting applications.
Photo courtesy of Christopher Hug, Dept. of Cell Biology, Washington
University Medical School
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Like
Molecular Dynamics' PhosphorImager systems, Storm
uses storage phosphor screens instead of film to capture quantitative data
from radioactive gels and blots. Technology from Kodak allows Molecular
Dynamics to offer a choice of screens to detect different isotopes. Screens
are sensitive to any source of ionizing radiation, including commonly used
isotopes such as 32P, 33P, 35S, 14C,
3H and 125I.
The
Storm system's wide exposure range and accurate signal quantitation
give you publication-ready data on the first exposure, plus greatly
simplified band intensity analysis -- even if you have both strong and weak
bands on the same exposure. And you can reduce your exposure times from
days to hours, or from hours to minutes, because
the PhosphorImager technology built into Storm is
many times faster than film.
Large
sample area for maximum applications versatility
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DNA Sequencing Gels
DNA
Sequencing gels and other large samples fit on the Storm system's 35 x 43
cm scan area. Storm offers the high resolution you need for DNA base
identification.
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Storm
has a 35 x 43 cm (14" x 17") sample area that accepts large
samples so you can scan sequencing-sized gels. Or, you can use the large
sample area to expose many small gels and blots simultaneously for maximum
throughput. Sample exposures take place in cassettes -- not in the
instrument -- so the Storm system is always available for scanning. With
the Windows NT operating system, scanning can continue even while you're
using the same computer to analyze your data and prepare your results for
presentation.
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Fig 2. Molecular Dynamics' proven storage phosphor screens
are available in 20x25cm and 35x43cm sequencing gel sizes. With proper
care, storage phosphor screens last indefinitely, regardless of how often
they are used. A ten minute exposure to visible light prepares the screen
for reuse.
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How storage phosphor works
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1.Exposure of the storage
phosphor screen to ionizing radiation induces latent image formation 2.
During laser scanning, the BaFBR:EU+2 crystals
in the screen release energy as blue light 3. and
return to ground state 4. Blue light is collected and measured to
form a quantitative representation of the sample.
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