Centrifugation

Our coverage of centrifugation will deal with the following topics, each of which is covered by the web tutorials indicated below.  A summary of key equations and topics is given here.  Refer to the web sites for further information.

I.  General Principles:    (lecture slides)

II.  Preparative Methods: 

Rotors - Course separations 

Density Gradient Methods

       Zonal Centrifugation - Sucrose gradient - Rate Method

       Isopycnic Methods - Equilibrium Method - Sep. by density (CsCl gradients)

III.  The Modern Analytical Ultracentrifuge

   See Beckman Review:  for a complete discussion of ultracentrifugation (Note pages 15-33).

Comparison of Optic Methods

IV.  Analytical Methods 

Sedimentation Velocity Experiments -

        PDF file on Sedimentation Velocity Methods from Beckman Instruments

The following slides are part of a tutorial on analytical ultracentrifugation Workshop slides.