CH370 -Syllabus and Links to Lecture Notes
| Date | Topic/ Link to Lecture Notes | Chapter | Articles |
| Aug. 29+ | Introduction: - Review of ionic properties of a.a. and proteins pKa, pI (slides) | 1 | |
| Sept. 3+ | Review Protein Structure: 2o, 3o, 4o / Protein Folding (slides) | 1,3,4 | ROSETTA |
| Sept. 5 | Properties of Nucleic Acids DNA Sequencing notes (ENCODE) (Genome Sequencing) Maxam Gilbert DNA Methods: sequencing and microarrays 1, 2, 3 (slides) | Pyroseq | |
| Sept. 10+ | Next Generation Sequencing Methods and Applications (slides) Impact | SystemsBiol | |
| Sept. 12* | Web resources: NCBI / Sequence Alignments (notes) / terms / BLAST comp tools PDB | NexGenRev | |
| Sept. 17 | Cloning and Expression HW-1 | EnzFunctInit | |
| Sept. 19 | Protein Purification - Isolation / (notes) (slides) | Expression Qiagen | |
| Sept. 24 | Chromatography - Catch-up & Review HW-1 due (KEY HW1) | Affinity ShigaTox | |
| Sept. 26 | *** EXAM I *** Review Summary NOTE: Exam in Rm WEL 2.224 KEY | -- | |
| Oct. 1 | Light and Matter Spectroscopy / Fluorescence FRET (notes) (Trp F) (tutorial) | 8,9,11 | FRET |
| Oct. 3 | Discovery of X-rays / Review of Radioactivity (Rontgen and X-rays) notes / slides | Tracer, TcImaging | |
| Oct. 8 | Electrophoresis IEF (primer on Protein Electrophoresis) DNA Electrophoresis On-line animations 1 2 slides | 2DElect | |
| Oct. 10 | Centrifugation / Sed. Velocity (s) BLAST Project due | 5 | |
| Oct. 15 | Sedimentation Equilibrium slides / applications HW-2 | 5,13 | Sed Equil |
| Oct. 17 | Light Scattering - static and dynamic slides / | 7 | LS_Ultracent |
| Oct. 22 | Circular Dichroism CD slides | 10 | MS_Mummy Proteins |
| Oct. 24 | Circular Dichroism continuted HW-2 due (KEY HW2) / Catch-up & Review | 15 | MALDI MSMS |
| Oct. 29 | **** EXAM II **** Review Summary NOTE: Exam in Rm WEL 2.224 KEY | ||
| Oct. 31 | Mass Spectroscopy MS intro slides Applications slides | Sys Biol | |
| Nov. 5** | Ligand Binding - Introduction / equations KJ / kinetics | 2,14 | |
| Nov. 7 | Ligand binding ITC / Surface Plasmon Res SPR (SPR1, SPR2 ) | 2,14 | MS_SPR_Xray |
| Nov. 12 | Intro. to the PDB, Image Formation slides | Microscopy | |
| Nov. 14 | X-ray Crystallography -1 notes | 6 | Xray |
| Nov. 19 | X-ray Crystallography -2 notes WC due | 6 | |
| Nov. 21 | Nuclear Magnetic Resonance - notes HW-3 | 12 | NMR |
| Nov. 26 | Nuclear Magnetic Resonance - | NMR | |
| Nov. 28 | ****** THANKSGIVING ****** | ||
| Dec 3 | Electron Microscopy - EM ; other imaging methods notes | EM, EM-2 | |
| Dec. 5 | Medical Imaging slides Review Summary / Student Reports HW-3 KEY | ||
| Dec. 11 | ***** EXAM III ***** (7-9 pm) (A-Mi in WEL 2.304 / Mo-Z WEL 2.256) KEY | ||
+Note – Guest lecturers Dr. Zhang 8/29 & 9/3 ; Dr. Hunicke-Smith 9/10
*September 13 - Last day to drop without possible academic penalty.
**November 5 - Last day undergraduates can drop a course for non-academic reasons, with the Dean's permission.
*HW-2: F - fv = m(dv/dt) solves to v = (F/f) [ 1 - exp(-ft/m)]. Calc. "s" for sed. velocity data and "M" from sedimentation equilibrium data.