BCH370 -Syllabus and Links to Lecture Notes  

Date	Topic/ Link to Lecture Notes	Chapter	Articles
Aug. 27	Introduction: - Review of  ionic properties of a.a. and proteins pKa, pI (slides)	1
Sept. 3	Review Protein Structure: 2^o, 3^o, 4^o ^/Protein Folding (slides)	1,3,4	ROSETTA
Sept. 8	Protein Purification  - Isolation   /   (notes)  (slides)
Sept. 10*	Chromatography		SystemsBiol
Sept. 15	Web resources: NCBI    / Sequence Alignments (notes) / terms / BLAST  comp tools PDB		NexGenRev
Sept. 17	Properties of Nucleic Acids   DNA Sequencing  notes  (ENCODE) (Genome Sequencing)  Maxam Gilbert  DNA sequencing and microarrays  1, 2, 3   (slides)  HW-1		EnzFunctInit Pyroseq
Sept. 22	Next Generation Sequencing Methods and Applications (slides)    Impact		Expression Qiagen
Sept. 24	Cloning and Expression   - (notes) (notes 2)		Affinity ShigaTox
Sept. 29	Light and Matter Spectroscopy  /  Fluorescence  FRET  (notes) Trp Fluor. HW-1 Due KEY	8,9,11	Fluor shifts
Oct. 1	**** EXAM 1 ****  WEL 2.122 KEY Exam 1 Summary
Oct. 6	X-rays and Radioactivity  notes Röntgen slides
Oct. 8	Electrophoresis Primer  Demos 1 2 slides	5	2DElect
Oct. 13	Centrifugation   Sedimentation vel.   slides	5
Oct. 15	Sedimentation Equilibrium  slides (see above)  /  applications     BLAST Project due (paper or online via Canvas / late after midnight)	5,13	Sed Equil
Oct. 20	Light scattering - static and dynamic   slides supp slides notes HW-2	7	LS_Ultracent
Oct. 22	Circular Dichroism CD slides	10	MS_Mummy Proteins
Oct. 27	Mass Spectroscopy MS intro slides	15	MALDI MSMS
Oct. 29	Mass spec applications  /  Review  HW-2 due (KEY)    Proteomics		Sys Biol
Nov. 3	**** EXAM II ****  Summary  NOTE:  Exam in BUR 108 (A-L) + BUR 216 (M-Z)  KEY Exam 2
Nov. 5**	Ligand Binding -   Introduction    / equations  / kinetics slides ITC	2, 14	MS_SPR_Xray
Nov. 10	Ligand binding    /  Surface Plasmon Res  SPR  (SPR1, SPR2 )	2, 14
Nov. 12	Intro. to the PDB, Image Formation slides		Microscopy
Nov. 17	X-ray Crystallography -1  notes part a HW-3	6	Xray
Nov. 19	X-ray Crystallography -2  notes part b  WC due	6
Nov.24	Nuclear Magnetic Resonance  - slides	12
Nov. 26	Nuclear Magnetic Resonance
Nov. 27/28	****  THANKSGIVING  ****
Dec . 1	Electron Microscopy - EM ; other imaging methods   slides Intro to EM Cryo EM		EM , EM-2
Dec. 3	Medical Imaging slides    HW-3 due   HW-3 KEY
Dec. 12	**** EXAM III ****   9-12   WEL 2.246    Exam 3 Rev Summary  Rev. 12/8 5-6 WEL 2.304  KEY

+Note – Guest lecturers Dr. Zhang 9/22; Dr. Hunicke-Smith 9/24 

*September 12 - Last day to drop without possible academic penalty.

**November 4 - Last day undergraduates can drop a course for non-academic reasons, with the Dean's permission.

*HW-2:   F - fv = m(dv/dt) solves to v = (F/f) [ 1 - exp(-ft/m)].  Calc. "s" for sed. velocity data and "M" from sedimentation equilibrium data.