CH370 -Syllabus and Links to Lecture Notes
| Date | Topic/ Link to Lecture Notes | Chapter | Other |
| Aug. 25+ | Introduction: Quiz - Review of ionic properties of a.a. and proteins pKa, pI | 1 | |
| Aug 30+ | Protein Structure: 2o, 3o, 4o Protein Folding | 1,3,4 | |
| Sept. 1 | Physical and Structural Properties of Nucleic Acids | ||
| Sept. 6 | DNA Sequencing notes | ||
| Sept. 8 | Introduction to NCBI / PDB Sequence Alignments (notes) / terms / BLAST comp tools | ||
| Sept. 13 | Protein Expression and Purification : Solubility / Chromatography (notes) HW-1 - due 9/20 | 5 | |
| Sept. 15 | Light and Matter Spectroscopy / Fluorescence FRET (notes) (Trp F) (tutorial) | 8,9,11 | |
| Sept. 20 | DNA Methods: - Review: HW-1 due (8:30) KEY HW-1 | ||
| Sept. 22* | *** EXAM I *** Review Summary NOTE: Exam in ART 1.102 EXAM I Key | -- | |
| Sept. 27 | DNA microarrays 1, 2, 3 / Applications | -- | |
| Sept 29 | Discovery of X-rays and Radioactivity (Rontgen and X-rays) notes / slides | ||
| Oct. 4 | Use of Radioisotopes and Counting (cont'd) | ||
| Oct. 6 | Electrophoresis IEF (primer on Protein Electrophoresis) DNA Electrophoresis On-line animations 1 2 BLAST due | 5 | |
| Oct. 11 | Centrifugation / Ultracentrifugation - Sed. Velocity (s) slides | 5,13 | |
| Oct. 13 | Sedimentation Equilibrium / applications HW-2 - due 10/25 | ||
| Oct. 18 | Light Scattering - static and dynamic slides / | 7 | |
| Oct. 20 | Circular Dichroism CD slides | 10 | |
| Oct. 25 | CD continued HW-2 due (8:30 am) KEY HW-2 | 15 | |
| Oct. 27 | ***** EXAM II **** Review Summary NOTE: Exam in ART 1.102 EXAM II Key | ||
| Nov. 1 | Mass Spectroscopy MS intro slides Applications slides / | ||
| Nov. 3 | Ligand Binding - Introduction / equations KJ / kinetics | 2,14 | |
| Nov. 8 | Ligand binding ITC / Surface Plasmon Res SPR (SPR1, SPR2 ) WC due | 2,14 | |
| Nov. 10 | Intro. to the PDB, Image Formation notes / | 6 | |
| Nov. 15 | X-ray Crystallography -1 notes HW-3 - due 11/22 | 6 | |
| Nov. 17 | X-ray Crystallography -2 notes | ||
| Nov. 22 | Nuclear Magnetic Resonance - notes /MRI / HW-3 due KEY HW-3 | 12 | |
| Nov. 24 | ****** THANKSGIVING ****** | ||
| Nov. 29 | ***** EXAM III *** Review Summary Note: Exam in ART 1.102 KEY EXAM-3 | ||
| Dec 1 | Electron Microscopy - EM ; other imaging methods notes - Review Wrap Up / Final Exam Format and Sample / Sample Ans sheet and equations given | ||
| (Comp. Final is required unless you have a grade of A or B) | |||
| Dec 10 | Comp. FINAL EXAM: 2:00 - 5:00 pm WEL 2.246 | ||
*September 22 - Last day to drop without possible academic penalty.
**October 19 - Last day undergraduates can drop a course for non-academic reasons, with the Dean's permission.
*HW-2: F - fv = m(dv/dt) solves to v = (F/f) [ 1 - exp(-ft/m)]. Calc. "s" for sed. velocity data and "M" from sedimentation equilibrium data.
+ Dr Zhang will substitute, Dr. Hackert will be
attending an international congress in Spain.