CH370 -Syllabus and Links to Lecture Notes  

Date Topic/ Link to Lecture Notes     Chapter  Other
Aug. 25+ Introduction: Quiz - Review of  ionic properties of a.a. and proteins  pKa, pI   1
Aug 30+ Protein Structure: 2o, 3o, 4o      Protein Folding   1,3,4
Sept. 1 Physical and Structural Properties of Nucleic Acids   
Sept. 6 DNA Sequencing  notes
Sept. 8 Introduction to NCBI    / PDB  Sequence Alignments (notes) / terms / BLAST   comp tools   
Sept. 13 Protein Expression and Purification :  Solubility / Chromatography     (notes)  HW-1 - due 9/20 5
Sept. 15 Light and Matter Spectroscopy  /  Fluorescence  FRET  (notes) (Trp F) (tutorial 8,9,11
Sept. 20 DNA Methods:    - Review:   HW-1 due  (8:30)     KEY HW-1
Sept. 22* *** EXAM I ***    Review Summary      NOTE:  Exam in ART 1.102    EXAM I Key --
Sept. 27 DNA microarrays 1, 2, 3 /  Applications --
Sept 29  Discovery of X-rays and Radioactivity  (Rontgen and X-rays) notes /  slides    
Oct. 4 Use of Radioisotopes and Counting (cont'd)        
Oct. 6 Electrophoresis   IEF   (primer on Protein Electrophoresis) DNA Electrophoresis On-line animations  1      BLAST due 5
Oct. 11 Centrifugation / Ultracentrifugation   - Sed. Velocity (s)  slides   5,13
Oct. 13 Sedimentation Equilibrium /  applications      HW-2 - due 10/25
Oct. 18 Light Scattering  - static and dynamic slides  /   7
Oct. 20  Circular Dichroism  CD slides 10
Oct. 25  CD continued   HW-2 due (8:30 am)   KEY HW-2 15
Oct. 27 ***** EXAM II **** Review Summary   NOTE:  Exam in ART 1.102     EXAM II Key
Nov. 1 Mass Spectroscopy MS intro   slides   Applications slides  /  
Nov. 3 Ligand Binding -   Introduction  / equations KJ / kinetics 2,14  
Nov. 8 Ligand binding  ITC  /  Surface Plasmon Res  SPR  (SPR1, SPR2 )   WC due     2,14  
Nov. 10 Intro. to the PDB,  Image Formation  notes /   6
Nov. 15  X-ray Crystallography -1  notes   HW-3 - due 11/22 6
Nov. 17  X-ray Crystallography -2  notes
Nov. 22  Nuclear Magnetic Resonance - notes  /MRI / HW-3 due  KEY HW-3 12  
Nov. 24      ****** THANKSGIVING ******
Nov. 29 ***** EXAM III ***  Review Summary  Note: Exam in ART 1.102 KEY EXAM-3   
Dec  1   Electron Microscopy - EM ; other imaging methods notes - Review Wrap Up /    Final Exam Format and Sample / Sample Ans sheet and equations given
(Comp. Final is required unless you have a grade of A or B)
Dec 10  Comp. FINAL EXAM:  2:00 - 5:00 pm   WEL 2.246
   

*September 22 - Last day to drop without possible academic penalty.

**October 19 - Last day undergraduates can drop a course for non-academic reasons, with the Dean's permission.

*HW-2:   F - fv = m(dv/dt) solves to v = (F/f) [ 1 - exp(-ft/m)].  Calc. "s" for sed. velocity data and "M" from sedimentation equilibrium data.

+ Dr Zhang will substitute, Dr. Hackert will be attending an international congress in Spain.